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 5c–d). CX-5461-mediated S33 phosphorylation of RPA was unbiased on the cell cycle stage and was not restricted towards the nucleoli in HR-deficient cells. Therefore, the development of ssDNA constructions in CX-5461 addressed cells may lead to replication fork stalling and ATR activation with HRD exacerbating CX-5461-mediated replication strain